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Sexually transmitted diseases quarterly report: genital warts and genital herpes simplex virus infections in England and Wales: 1994. CDR Weekly. 1995; 5: 18688. Benedetti J, Corey L, Ashley R. Recurrence rates in genital herpes after symptomatic first-episode infection. Ann Int Med 1994; 121: 84754. Lafferty W E, Coombs R W, Benedetti J, Critchlow C, Corey L. Recurrences after oral and genital herpes simplex virus infection: influence of anatomic site and viral type. N Engl J Med 1987; 316: 144449. Breinig M K, Kingsley L A, Armstrong J A, Freeman D J, Ho M. Epidemiology of genital herpes in Pittsburgh: Serologic, sexual, and racial correlates of apparent and inapparent herpes simplex infections. J Infect Dis 1990; 162: 299 Gibson J J, Hornung C A, Alexandra G R, Lee F K, Potts W A, Nahmias A J. A cross-sectional study of herpes simplex virus types 1 and 2 in college students: Occurrence and determinants of infection. J Infect Dis 1990; 162: 30612. Stavraky K M, Rawls W E, Chiavetta J, Donner A P, Wanklin J M. Sexual and socioeconomic factors affecting the risk of past infections with herpes simplex virus type 2. J Epidemiol 1983; 118: 10921. Koutsky L A, Stevens C E, Holmes K K et al. Underdiagnosis of genital herpes by current clinical and viral-isolation procedures. New Engl J Med 1992; 326: 153339. Langenberg A, Benedetti J, Jenkins J, Ashley R, Winter C, Corey L. Development of clinically recognizable genital lesions among women previously identified as having `asymptomatic' herpes simplex virus type 2 infection. Ann Intern Med 1989; 110: 88287. Whitley J. Herpes simplex virus infections of women and their offspring: Implications for a developed society. Proc Natl Acad Sci USA 1994; 91: 244147. Luby E D, Klinge V. Genital herpes: a pervasive psychosocial disorder. Arch Dermatol 1985; 121: 49497. Van der Plate C, Aral S O. Psychological aspects of genital herpes virus infection. Health Psychol 1987; 6: 5772. Carney O, Ross E, Bunker C, Ikkos G, Mindel A. A prospective study of the psychological impact on patients with a first-episode of genital herpes. Genitourin Med 1994; 70: 4045. Goldmeier D, Johnson A, Byrne M, Barton S. Psychosocial implications of recurrent genital herpes simplex virus infection. Genitourin Med 1988; 64: 32730. Fenemma J S A, van Ameijden E J C, Coutinho R A, van den Hoek A J A HIV, sexually transmitted diseases and gynaecologic disorders in women: increased risk for genital herpes and warts among HIV-infected prostitutes in Amsterdam. AIDS 1995; 9: 107178. Bagdades E, Pillay D, Squire S et al. Relationship between herpes simplex virus ulceration and CD4 + cell counts in patients with HIV infection. AIDS 1992; 6: 1317. Centers for Disease Control: Revised classification system for HIV infection and expanded surveillance case definition for AIDS among adolescents and adults. MMWR 1992; 41: 15. Augenbraun M H, Feldman J, Chirgwin K et al. Increased genital shedding of herpes simplex virus type 2 in HIVseropositive women. Ann Intern Med 1995; 123: 84547. Kreis J, Caral M, Meheus A. Rle of sexually transmitted diseases in transmitting human immunodeficiency virus. Genitourin Med 1988; 64: 12.
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FIG. 6. Time-course analyses of HPP and RHPP accumulations in MDCK cells that overexpress hOCT1, hOCT2, or hOCT3. Either 10 M HPP A ; or 10 RHPP B ; was applied to the apical side of the monolayer of MDCK cells that overexpress hOCT1, hOCT2, or hOCT3, and the cells were incubated for 0, 5, 10, 15, or 90 min at 37C. The cellular concentrations of HPP and RHPP were determined by LC MS analysis. Exponential saturation curves were fitted to the experimental data. Linear coefficient r2 0.9990. The data are presented as arithmetic mean S.E.M. n 3 ; . , 0.01, relative to the mock-treated cells. FIG. 5. [3H]MPP and [14C]TEA concentrations in MDCK cells that stably express the hOCT1, hOCT2, and hOCT3 proteins. Cells were incubated for 30 min at 37C with 200 nM [3H]MPP A ; and [14C]TEA B ; applied to the apical cell border. The data are represented as arithmetic mean S.E.M. n 3 ; . , 0.01. C, Western blot analysis of MDCK cells that stably express hOCT1, hOCT2, and hOCT3. The hOCT1, hOCT2, and hOCT3 proteins have molecular sizes in the 50 75-kDa range.
Fig. 2. Effect of OTC on ROS levels in BAL fluids of ovalbumin-sensitized and -challenged mice. Sampling was performed at 72 h after the last challenge in salineinhaled mice administered saline SAL SAL ; , ovalbumin-inhaled mice administered saline OVA SAL ; , ovalbumin-inhaled mice administered drug vehicle OVA VEH ; , ovalbumin-inhaled mice administered 80 mg kg OTC OVA OTC 80 ; , and ovalbumin-inhaled mice administered 160 mg kg OTC OVA OTC 160 ; . AD, representative fluorescence microscopy shows ROS-positive cells in the BAL fluids. E, dichlorofluorescein fluorescence intensity is presented as the relative ratio of ROS levels in ovalbumin-inhaled mice administered saline OVA SAL ; , ovalbumin-inhaled mice administered drug vehicle OVA VEH ; , ovalbumin-inhaled mice administered 80 mg kg OTC OVA OTC 80 ; , and ovalbumin-inhaled mice administered 160 mg kg OTC OVA OTC 160 ; . The relative ratio of ROS levels in the BAL fluids of SAL SAL is arbitrarily presented as 100. Bars represent mean S.E.M. from six independent experiments. * , p 0.05. OVA SAL and OVA VEH versus SAL SAL; , p 0.05. OTC treatment groups versus OVA SAL and cutivate.
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